EJEMPLOS DE OXIDORREDUCTASAS PDF

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Algunas enzimas que contienen al cofactor de molibdeno son la xantina oxidasaDMSO reductasasulfito oxidasa y nitrato reductasa. Tools for Biotechnological Processes”, Biomolecules, 4, pp. Ketoreductase polypeptides for the production of a 3-arylhidroxipropanamina from a 3-arylcetopropanamina.

Methods and compositions for treating cardiovascular disease using,,,oxidorredudtasas,,,,,,,,,,,,, or Candida oxidoreductase nemodendra presents an equal activity against Rbutanol, 2-propanol and 2-octanol-R, also has the enzyme also approximately equal activity against two-octanone. Process for the enantioselective enzymatic reduction of a keto compound to give the corresponding chiral hydroxy, wherein the ketone compound is reduced ejemploa the presence of a cofactor with an oxidoreductase, wherein an oxidoreductase which is selected from the group consisting used for oxidoreductases for ejemploz a encodes the nucleic acid sequence SEQ ID NO: Accordingly absent information sequence on the 5 ‘end of the gene is obtained.

Enzimas: aceleradores de reacciones químicas en las células y en la industria

Tabla 1 Table 1. The main feasibility of using biocatalysts for reduction of ketone compounds prochiral to give compounds of chiral hydroxyl demonstrated in the past repeatedly using model ejemplls, and the conference both oxidoreductases isolated as different systems of biotransformation of whole cells.

NADH dependent alcoholdeshidrogenasas suitable obtainable, for example bakers yeast, Candida boidinii, Candida parapsilosis or Pichia capsulata. Cosubstrate are used in this respect preferably primary and secondary, such as ethanol, 2-propanol, 2-butanol, 2-pentanol, 3-pentanol, 4-methylpentanol, 2-heptanol, 2-octanol or cyclohexanol alcohols.

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Chem57, Este tipo de empaques se ha utilizado para mantener las propiedades sensoriales de quesos y mayonesa durante su almacenamiento BANKAR et al. Los cosustratos adecuados para estas alcoholdeshidrogenasas oxidorreductaass los alcoholes secundarios ya mencionados tales como etanol, 2propanol isopropanol2-butanol, 2-pentanol4-metilpentanol, 2-octanol o ciclohexanol.

Continuous asymmetric ketone reduction processes with recombinant Escherichia coli. Generally, these enzymes dependent cofactors. The antibodies may be monoclonal or polyclonal. To this end the various buffers 50 mM were set in the range of pH 4 to 11 and oxidoreductases prepared according to example 4 were diluted with. In this respect the biocatalytic approach proved advantageous with respect to essentially mild reaction conditions, and absent byproducts obtainable with essentially enantiomeric excesses better frequency.

The invention further relates to a recombinant host cell is a bacterial, yeast, insect, plant or mammalian and transformed or transfected with an expression vector of oxidorrreductasas type and to a preparation process for obtaining a carbonyl reductase which is based on culturing a recombinant host cell of this type. Los cosustratos adecuados de la formiato Suitable cosubstrates of formate.

Reaction mixture for the selection of activity: The solvent may also serve in this respect as cosubstrate simultaneously cofactor regeneration. Biphenylyl residues, naphthyl residues and, in particular phenyl radicals are preferred aryl radicals. Reductions ketone compounds prochiral to give hydroxy compounds and conversely occur in nature in many biochemical pathways, both the primary metabolism and secondary metabolism, in any organism and are catalyzed by different types of secondary and oxidoreductases alcoholdeshidrogenasas.

It is also suitable for the reduction of 4-haloacetoacetato ester acid esters Rhalohydroxybutyric acid. Compositions and methods for producing stereoisomerically pure statins and synthetic intermediates therefor.

The reaction mixtures for ee determination were extracted after 24 hours h for example with chloroform and by gas chromatography GC the enantiomeric excess was determined. The reaction conditions are essentially the same as in the aforementioned method for the enantiospecific reduction of the ketone compound of formula I. Por regla general, estas enzimas dependen de cofactores.

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¿Enzimas en los alimentos? Bioquímica de lo comestible

Las molibdopterinas oxidorredutcasas una clase de cofactores hallados en casi todas las enzimas que contienen molibdeno y en todas las que contienen tungsteno. Por lo tanto, las molibdopterinas pueden formar complejos tanto con molibdeno como con tungsteno, por ejemplo, en las bacterias. Partially can also dispensed organic solvent itself, then the organic phase by the ketone compound to be reduced DEDE Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products.

El segmento de ADN que codifica la respectiva oxidorreductasa se amplifica con ayuda de polimerasa Platinum pfx Invitrogen.

For this, the aqueous phase is separated, the organic phase is filtered. Of the two enantiomers of 2-octanol preferentially oxidizes Soctanol. The enantiomeric excess oxidoreductasas calculated as follows: Definition of enzyme units: The reaction was followed 10 for 1 min at nm.

American Association of Cereal Chemists,pp. Examples Ry oxidoreductases and dehydrogenase S-specific suitable high enantioselectivity: Para el aislamiento de las oxidorreductasas microbianas dependientes de NAD P H se cultivaron los microorganismos tal como se ha descrito en el ejemplo 1. Alcohol dehydrogenase and its use for the enzymatic preparation of chiral hydroxy compounds. Las secuencias codificantes y no codificantes en extremo 5′ terminal se determinan con ayuda del sistema 5′-RACE Invitrogen.